tuj1 covance Search Results


rabbit anti tuj1  (Covance)
86
Covance rabbit anti tuj1
a, Experimental scheme for generating induced neuronal (iN) cells differentiated from human embryonic stem cells (hESCs) or neural progenitor cells (NPCs) by overexpression of the neuronal gene, Ngn2. b, Brightfield and fluorescent images of iN cells stained for the neural cell marker <t>Tuj1</t> (red), pluripotency marker Sox2 (red), cell proliferation marker Ki67 (green) from wt hESC-derived iN cells and mutant hESC-derived iN cells after 2 weeks of differentiation. Data are from at least three independent experiments. Scale bars, 50 μm. c, Neurite length in cells were measured at 12 days of iN differentiation (*p < 0.01). d, Quantitative RT-PCR analysis of neural cell marker Map2 expression level at 7 days of hESC differentiation. Two-tailed t-test, Mean ± SD (n=3, *p < 0.01). e, Brightfield and fluorescent images of NPC-derived iN cells stained for the neural cells marker Tuj1 (green) from IDH1-wt NPC-derived iN cells and IDH1-R132H mutation NPC-derived iN cells after 2 weeks of differentiation. f, Quantitative RT-PCR analysis of neural cell marker Map2 expression at 7 days of NPC differentiation. Values represent mean ± SD (n=3, *p < 0.01). g, Cell number counting of Ki67 positive wt hESC-derived iN cells and mutant hESC-derived iN cells at 10 days of differentiation (*p < 0.01). h, Cell number counting of Tuj1 positive wt hESC-derived iN cells and mutant hESC-derived iN cells at 10 days of differentiation (*p < 0.01). i, Cell number counting of Sox2 positive wt hESC-derived iN cells and mutant hESC-derived iN cells at 10 days of differentiation. Values represent the number of cells showing positive in each situation ± SD (n=3, *p < 0.01).
Rabbit Anti Tuj1, supplied by Covance, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tuj1+covance/anti+tuj1/bio_rxiv__2025__04__22__649974-241-12-14
Average 86 stars, based on 1 article reviews
rabbit anti tuj1 - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier
mouse anti tuj 1  (Covance)
86
Covance mouse anti tuj 1
a, Experimental scheme for generating induced neuronal (iN) cells differentiated from human embryonic stem cells (hESCs) or neural progenitor cells (NPCs) by overexpression of the neuronal gene, Ngn2. b, Brightfield and fluorescent images of iN cells stained for the neural cell marker <t>Tuj1</t> (red), pluripotency marker Sox2 (red), cell proliferation marker Ki67 (green) from wt hESC-derived iN cells and mutant hESC-derived iN cells after 2 weeks of differentiation. Data are from at least three independent experiments. Scale bars, 50 μm. c, Neurite length in cells were measured at 12 days of iN differentiation (*p < 0.01). d, Quantitative RT-PCR analysis of neural cell marker Map2 expression level at 7 days of hESC differentiation. Two-tailed t-test, Mean ± SD (n=3, *p < 0.01). e, Brightfield and fluorescent images of NPC-derived iN cells stained for the neural cells marker Tuj1 (green) from IDH1-wt NPC-derived iN cells and IDH1-R132H mutation NPC-derived iN cells after 2 weeks of differentiation. f, Quantitative RT-PCR analysis of neural cell marker Map2 expression at 7 days of NPC differentiation. Values represent mean ± SD (n=3, *p < 0.01). g, Cell number counting of Ki67 positive wt hESC-derived iN cells and mutant hESC-derived iN cells at 10 days of differentiation (*p < 0.01). h, Cell number counting of Tuj1 positive wt hESC-derived iN cells and mutant hESC-derived iN cells at 10 days of differentiation (*p < 0.01). i, Cell number counting of Sox2 positive wt hESC-derived iN cells and mutant hESC-derived iN cells at 10 days of differentiation. Values represent the number of cells showing positive in each situation ± SD (n=3, *p < 0.01).
Mouse Anti Tuj 1, supplied by Covance, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tuj1+covance/1+anti+mouse+tuj/schreck_karisa_colleen__2012__notch_signaling_in_brain_development_and_cancer-2156-0-4
Average 86 stars, based on 1 article reviews
mouse anti tuj 1 - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

Image Search Results


a, Experimental scheme for generating induced neuronal (iN) cells differentiated from human embryonic stem cells (hESCs) or neural progenitor cells (NPCs) by overexpression of the neuronal gene, Ngn2. b, Brightfield and fluorescent images of iN cells stained for the neural cell marker Tuj1 (red), pluripotency marker Sox2 (red), cell proliferation marker Ki67 (green) from wt hESC-derived iN cells and mutant hESC-derived iN cells after 2 weeks of differentiation. Data are from at least three independent experiments. Scale bars, 50 μm. c, Neurite length in cells were measured at 12 days of iN differentiation (*p < 0.01). d, Quantitative RT-PCR analysis of neural cell marker Map2 expression level at 7 days of hESC differentiation. Two-tailed t-test, Mean ± SD (n=3, *p < 0.01). e, Brightfield and fluorescent images of NPC-derived iN cells stained for the neural cells marker Tuj1 (green) from IDH1-wt NPC-derived iN cells and IDH1-R132H mutation NPC-derived iN cells after 2 weeks of differentiation. f, Quantitative RT-PCR analysis of neural cell marker Map2 expression at 7 days of NPC differentiation. Values represent mean ± SD (n=3, *p < 0.01). g, Cell number counting of Ki67 positive wt hESC-derived iN cells and mutant hESC-derived iN cells at 10 days of differentiation (*p < 0.01). h, Cell number counting of Tuj1 positive wt hESC-derived iN cells and mutant hESC-derived iN cells at 10 days of differentiation (*p < 0.01). i, Cell number counting of Sox2 positive wt hESC-derived iN cells and mutant hESC-derived iN cells at 10 days of differentiation. Values represent the number of cells showing positive in each situation ± SD (n=3, *p < 0.01).

Journal: bioRxiv

Article Title: Oncogenic mutations disrupt lineage differentiation via epigenetic perturbations

doi: 10.1101/2025.04.22.649974

Figure Lengend Snippet: a, Experimental scheme for generating induced neuronal (iN) cells differentiated from human embryonic stem cells (hESCs) or neural progenitor cells (NPCs) by overexpression of the neuronal gene, Ngn2. b, Brightfield and fluorescent images of iN cells stained for the neural cell marker Tuj1 (red), pluripotency marker Sox2 (red), cell proliferation marker Ki67 (green) from wt hESC-derived iN cells and mutant hESC-derived iN cells after 2 weeks of differentiation. Data are from at least three independent experiments. Scale bars, 50 μm. c, Neurite length in cells were measured at 12 days of iN differentiation (*p < 0.01). d, Quantitative RT-PCR analysis of neural cell marker Map2 expression level at 7 days of hESC differentiation. Two-tailed t-test, Mean ± SD (n=3, *p < 0.01). e, Brightfield and fluorescent images of NPC-derived iN cells stained for the neural cells marker Tuj1 (green) from IDH1-wt NPC-derived iN cells and IDH1-R132H mutation NPC-derived iN cells after 2 weeks of differentiation. f, Quantitative RT-PCR analysis of neural cell marker Map2 expression at 7 days of NPC differentiation. Values represent mean ± SD (n=3, *p < 0.01). g, Cell number counting of Ki67 positive wt hESC-derived iN cells and mutant hESC-derived iN cells at 10 days of differentiation (*p < 0.01). h, Cell number counting of Tuj1 positive wt hESC-derived iN cells and mutant hESC-derived iN cells at 10 days of differentiation (*p < 0.01). i, Cell number counting of Sox2 positive wt hESC-derived iN cells and mutant hESC-derived iN cells at 10 days of differentiation. Values represent the number of cells showing positive in each situation ± SD (n=3, *p < 0.01).

Article Snippet: The following primary antibodies with indicated dilution in blocking buffer were used: Rabbit anti-Tuj1 (Covance, BioLegend, MRB-435P, 1:1,000), Mouse anti-Tuj1 (Covance, BioLegend, MMS-435P, 1:1,000), Rabbit anti-human Sox1 (Millipore, AB15766, 1:1000), Mouse anti-Tra-1-60 (Millipore, MAB4360, 1:1000), Rabbit anti-Ki67 (Abcam, ab15580, 1:1000), Goat anti-Sox2 (Santa Cruz, Y-17, sc-17320, 1:1000), Mouse anti-Sox2 (R&D, MAB2018, 1:200), Goat anti-Sox2 (R&D, AF2018, 1:200), Mouse anti-Oct-3/4 (Sata Cruz, C-10, sc-5279), Mouse anit-Nestin (DSHB, rat-401, 1:200), Mouse anit-Nestin (R&D, MAB1259, 1:200), Rabbit anti-ASCL1 (Abcam, ab74065, 1:200).

Techniques: Over Expression, Staining, Marker, Derivative Assay, Mutagenesis, Quantitative RT-PCR, Expressing, Two Tailed Test

a, Cell number counting of wt hESCs and mutant hESCs after 3 days of dox-induction (n=3, NS: p > 0.05). b, Cell number counting of wt hESC-derived iN cells and mutant hESC-derived iN cells at 7 days of differentiation (n=3, *p < 0.01). c, The ratio of the Sox2 positive hESC-derived iN cell number and Tuj1 positive hESC-derived iN cell number at 10 days of differentiation (*p < 0.01).

Journal: bioRxiv

Article Title: Oncogenic mutations disrupt lineage differentiation via epigenetic perturbations

doi: 10.1101/2025.04.22.649974

Figure Lengend Snippet: a, Cell number counting of wt hESCs and mutant hESCs after 3 days of dox-induction (n=3, NS: p > 0.05). b, Cell number counting of wt hESC-derived iN cells and mutant hESC-derived iN cells at 7 days of differentiation (n=3, *p < 0.01). c, The ratio of the Sox2 positive hESC-derived iN cell number and Tuj1 positive hESC-derived iN cell number at 10 days of differentiation (*p < 0.01).

Article Snippet: The following primary antibodies with indicated dilution in blocking buffer were used: Rabbit anti-Tuj1 (Covance, BioLegend, MRB-435P, 1:1,000), Mouse anti-Tuj1 (Covance, BioLegend, MMS-435P, 1:1,000), Rabbit anti-human Sox1 (Millipore, AB15766, 1:1000), Mouse anti-Tra-1-60 (Millipore, MAB4360, 1:1000), Rabbit anti-Ki67 (Abcam, ab15580, 1:1000), Goat anti-Sox2 (Santa Cruz, Y-17, sc-17320, 1:1000), Mouse anti-Sox2 (R&D, MAB2018, 1:200), Goat anti-Sox2 (R&D, AF2018, 1:200), Mouse anti-Oct-3/4 (Sata Cruz, C-10, sc-5279), Mouse anit-Nestin (DSHB, rat-401, 1:200), Mouse anit-Nestin (R&D, MAB1259, 1:200), Rabbit anti-ASCL1 (Abcam, ab74065, 1:200).

Techniques: Mutagenesis, Derivative Assay

a, The morphology of U251 cells. U251 cell line is an IDH1 mutation glioblastoma cell line. Scale bars, 25 μm. b, c, Brightfield and fluorescent images of immunostaining TRA-1-60 (red) of U251 cells at 14 days reprogramming with OSKM for colony identification. d, e, Brightfield and fluorescent images of immunostaining U251 cells at 14 days differentiation by Ngn2 overexpression for the neural cells marker Tuj1 (green). f, The morphology of K562 cells. K562 cell line is an IDH1 mutant leukemia cell line. g, h, Brightfield and fluorescent images of K562 cells immunostaining for TRA-1-60 (green) at 14 days reprogramming with OSKM. No TRA-1-60 positive staining colony. i, j, Brightfield and fluorescent images of staining Tuj1 (red) and DAPI (blue) on K562 cells at 14 days differentiation by Ngn2 overexpression. k, The morphology of HCT116 cells. HCT116 cell line is an IDH1 mutant colon cancer cell line. l, m, Brightfield and fluorescent images of staining TRA-1-60 (green) and DAPI (blue) on HCT116 cells at 14 days reprogramming with OSKM. HCT116 cells cluster on feeders and grow more as colony-like clumps as shown in the photo. All cells are TRA-1-60 positive. n, o, Brightfield and fluorescent images of staining Tuj1 (red) and DAPI (blue) on HCT116 cells at 14 days differentiation by Ngn2 overexpression. p, q, r, s, Immunostaining U251 cells with the pluripotency marker Sox2 (red), neural stem/progenitor cell markers Nestin (green) and ASCL1 (red), and neural cell marker Tuj1 (green).

Journal: bioRxiv

Article Title: Oncogenic mutations disrupt lineage differentiation via epigenetic perturbations

doi: 10.1101/2025.04.22.649974

Figure Lengend Snippet: a, The morphology of U251 cells. U251 cell line is an IDH1 mutation glioblastoma cell line. Scale bars, 25 μm. b, c, Brightfield and fluorescent images of immunostaining TRA-1-60 (red) of U251 cells at 14 days reprogramming with OSKM for colony identification. d, e, Brightfield and fluorescent images of immunostaining U251 cells at 14 days differentiation by Ngn2 overexpression for the neural cells marker Tuj1 (green). f, The morphology of K562 cells. K562 cell line is an IDH1 mutant leukemia cell line. g, h, Brightfield and fluorescent images of K562 cells immunostaining for TRA-1-60 (green) at 14 days reprogramming with OSKM. No TRA-1-60 positive staining colony. i, j, Brightfield and fluorescent images of staining Tuj1 (red) and DAPI (blue) on K562 cells at 14 days differentiation by Ngn2 overexpression. k, The morphology of HCT116 cells. HCT116 cell line is an IDH1 mutant colon cancer cell line. l, m, Brightfield and fluorescent images of staining TRA-1-60 (green) and DAPI (blue) on HCT116 cells at 14 days reprogramming with OSKM. HCT116 cells cluster on feeders and grow more as colony-like clumps as shown in the photo. All cells are TRA-1-60 positive. n, o, Brightfield and fluorescent images of staining Tuj1 (red) and DAPI (blue) on HCT116 cells at 14 days differentiation by Ngn2 overexpression. p, q, r, s, Immunostaining U251 cells with the pluripotency marker Sox2 (red), neural stem/progenitor cell markers Nestin (green) and ASCL1 (red), and neural cell marker Tuj1 (green).

Article Snippet: The following primary antibodies with indicated dilution in blocking buffer were used: Rabbit anti-Tuj1 (Covance, BioLegend, MRB-435P, 1:1,000), Mouse anti-Tuj1 (Covance, BioLegend, MMS-435P, 1:1,000), Rabbit anti-human Sox1 (Millipore, AB15766, 1:1000), Mouse anti-Tra-1-60 (Millipore, MAB4360, 1:1000), Rabbit anti-Ki67 (Abcam, ab15580, 1:1000), Goat anti-Sox2 (Santa Cruz, Y-17, sc-17320, 1:1000), Mouse anti-Sox2 (R&D, MAB2018, 1:200), Goat anti-Sox2 (R&D, AF2018, 1:200), Mouse anti-Oct-3/4 (Sata Cruz, C-10, sc-5279), Mouse anit-Nestin (DSHB, rat-401, 1:200), Mouse anit-Nestin (R&D, MAB1259, 1:200), Rabbit anti-ASCL1 (Abcam, ab74065, 1:200).

Techniques: Mutagenesis, Immunostaining, Over Expression, Marker, Staining